Journal: bioRxiv

Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments

doi: 10.1101/2022.05.06.490916

Figure Lengend Snippet: (A) Schematic representation of the experimental timeline. Grey (SN) and pink (MSEW) lines represent (B) the percentage of females that retrieved the first pup during the test. (C) Proportion of females that retrieved (filled bar) all pups. (D) Number of pups that were retrieved. Representative photographs of nests built by SN (F) and MSEW (G) mothers. (E) Dispersion of the pups by their respective dams is shown as the occupied area of the nest (H) and the average distance of the pups from the centroid from each litter (X mean ; I) of SN and MSEW groups. (J) Time spent digging the sawdust. (K) Immobility time spent in TST and (L) self-grooming behaviour on splash test. (M) Content of allopregnanolone in plasma samples of SN and MSEW dams. pTRKB and BDNF protein levels in PFC (N,O) and hippocampus (P,Q). All data are represented as the mean ± SEM. (Log-rank test * p < 0.05; Fischer’s test *** p < 0.001; t-Student’s test, * p < 0.05, *** p < 0.001, Mann-Whitney’s test *** p < 0.001). MS, maternal separation; MSEW, maternal separation with early weaning; PD, postpartum day; PFC, prefrontal cortex; PRT, pup retrieval test; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; W, day of weaning day.

Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for allopregnanolone determination (DetectXs Allopregnanolone Enzyme; Immunoassay Kit K044-H1 Arbor Assays, United States).

Techniques:

Journal: bioRxiv

Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments

doi: 10.1101/2022.05.06.490916

Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and pink (MSEW) dots represent (B) the time that animals are immobile in TST after vehicle, ketamine or allopregnanolone treatments (n = 10-12 / group). (C) Graph shows the cumulative time self-grooming behaviour of SN (grey dots) and MSEW (pink dots) dams in splash test after vehicle, ketamine or allopregnanolone treatments (n = 10-12 / group). All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, ** p < 0.01, *** p < 0.001). Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; perf, perfusion; PD, post-partum day; PRT, pup retrieval test; PW, post-weaning day; SN, standard nest; SPT, splash test; TST, tail suspension test; W, day of weaning day.

Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for allopregnanolone determination (DetectXs Allopregnanolone Enzyme; Immunoassay Kit K044-H1 Arbor Assays, United States).

Techniques:

Journal: bioRxiv

Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments

doi: 10.1101/2022.05.06.490916

Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and pink (MSEW) dots represent (B) the average of BrdU-positive cells per hemisphere after vehicle, ketamine or allopregnanolone treatments (n = 5-6 / group). Representative of single stack of BrdU (green cells) and NeuN-BrdU merge of vehicle- (images of SN female are C and C’; images of MSEW female are D and D’), ketamine- (images of SN female are E and E’; images of MSEW group are F and F’) and allopregnanolone-treated animals (images of SN female are G and G’; images of MSEW female are H and H’). All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, *** p < 0.001). Scale bar 100 μm. Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; Perf, perfusion; PD, postpartum day; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; W, day of weaning day.

Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for allopregnanolone determination (DetectXs Allopregnanolone Enzyme; Immunoassay Kit K044-H1 Arbor Assays, United States).

Techniques:

Journal: bioRxiv

Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments

doi: 10.1101/2022.05.06.490916

Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and white (MSEW) bars represent the average number of (B) VGLUT1-, (C) VGAT-positive puncta in IL and (D) excitatory/inhibitory ratio (n = 5-6 / group) after vehicle, ketamine or allopregnanolone treatments measured in two different confocal z-planes in both hemispheres of each individual animal. Representative single confocal planes of puncta expressing excitatory (green, VGLUT1, E-J) and inhibitory (red, VGAT, E’-J’) synaptic markers in IL. All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, *** p < 0.001). Scale bar 50 μm. Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; Perf, perfusion; PD, postpartum day; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; VGAT, vesicular GABA transporter; VGLUT1, vesicular glutamate transporter type 1W, day of weaning day.

Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for allopregnanolone determination (DetectXs Allopregnanolone Enzyme; Immunoassay Kit K044-H1 Arbor Assays, United States).

Techniques: Expressing

Journal: JACC: Basic to Translational Science

Article Title: Estrogen Receptor-α Non-Nuclear Signaling Confers Cardioprotection and Is Essential to cGMP-PDE5 Inhibition Efficacy

doi: 10.1016/j.jacbts.2019.12.009

Figure Lengend Snippet: Cardiac Phenotype of KRR KI/KI Mouse and PDE5i Efficacy Cardiac echocardiography assessed with (A) the percentage of fractional shortening (FS) and (B) end-diastolic dimension (Dd) (mm) at 3 weeks. (C) Heart weight (HW) (mg) normalized to tibial length (TL) (mm) at 3 weeks after transverse aortic constriction (TAC). (D) Effect of a phosphodiesterase 5 inhibitor (PDE5i) on percentage of FS time course after TAC (n = 5 to 8 per group) and (E) HW/TL (mg/mm) at 3 weeks (n = 6 to 8 per group). E2 = estradiol, KRR KI/KI = KRR knock-in mutant ERα; KRR WT/WT = wild-type littermate; OVx = ovariectomy.

Article Snippet: Serum and heart E2 levels were measured using an E2 enzyme immunoassay kit (Arbor Assays Estradiol Serum EIA kit [Arbor Assays, Inc., Ann Arbor, Michigan]) according to the manufacturer protocols.

Techniques: Knock-In, Mutagenesis